Histatin-1 is an endogenous ligand of the sigma-2 receptor
By Kyung-No Son, Hyun Lee, Dhara Shah, Sushma Kalmodia, Ryan Cree Miller, Marwan Ali, Arun Balasubramaniam, Stephanie M. Cologna, Hyunjoon Kong, Deepak Shukla, and Vinay Kumar Aakalu
Excerpt from the manuscript published in final edited form in FEBS J. 2021 Jul 7 : 10.1111/febs.16108. DOI: 10.1111/febs.16108
Editor’s Highlights
- The Sigma-2 receptor (S2R) was recently identified as the endoplasmic reticular protein, Transmembrane Protein 97 (TMEM97), implicated heavily in cancer and neurodegenerative diseases.
- Belonging to the endogenous antimicrobial peptide (AMP) family, Histatins are overexpressed in multiple cancer cell types and may modulate the effects of epithelial-mesenchymal transition (EMT) factors in these cancers.
- Histatin-1 (Hst1), is thought to be the primary wound healing agent in saliva and is known to promote cell migration and modulate cellular adhesion in multiple cell types.
- Hst1 is an endogenous ligand for TMEM97 and TMEM97 is a mammalian receptor for Hst1.
Abstract
The Sigma-2 receptor (S2R) (a.k.a TMEM97) is an important endoplasmic reticular protein involved in cancer, cholesterol processing, cell migration, and neurodegenerative diseases, including Niemann–Pick Type C. While several S2R pharmacologic agents have been discovered, its recent (2017) cloning has limited biological investigation, and no endogenous ligands of the S2R are known. Histatins are a family of endogenous antimicrobial peptides that have numerous important effects in multiple biological systems, including antifungal, antibacterial, cancer pathogenesis, immunomodulation, and wound healing. Histatin-1 (Hst1) has important roles in epithelial wound healing and cell migration, and is the primary wound healing agent in saliva. Little is understood about the downstream machinery that underpins the effects of histatins, and no mammalian receptor is known to date. In this study, we show, using biophysical methods and functional assays, that Hst1 is an endogenous ligand for S2R and that S2R is a mammalian receptor for Hst1.
Introduction
The Sigma-2 receptor (S2R) was recently identified as the endoplasmic reticular protein, Transmembrane Protein 97 (TMEM97) [1]. S2R, implicated heavily in cancer and neurodegenerative diseases, was originally described pharmacologically, with disparate small molecules and drugs found to target S2R. These agents are active in multiple areas including cancer, pain, Alzheimer’s disease, aging and mitochondrial disorders, and multiple sclerosis [2]. TMEM97 is also known to be important in cancer cell metastasis, cellular migration and has a described interaction with the Niemann–Pick Type C (NPC) intracellular cholesterol transporter 1 (NPC1) protein [1,2]. NPC1 is deficient, or defective, in the vast majority of patients with Niemann–Pick Type C disease, and is critical for cholesterol processing and distribution [3–5]. While numerous actions and pharmacologic modulators have been tied to TMEM97/S2R, to our knowledge, no well-described endogenous ligand is known [1,2].
Belonging to the endogenous antimicrobial peptide (AMP) family, histatins were originally thought to be antifungal components of saliva [6]. In the last decade, numerous roles for histatins have been described in multiple epithelial and nonepithelial systems, including antiviral, antibacterial, wound healing, and immunomodulatory actions [7–9]. Histatins, particularly histatin-1 (Hst1), are thought to be the primary wound healing agents in saliva and are known to promote cell migration and modulate cellular adhesion in multiple cell types [7–9]. Histatins are overexpressed in multiple cancer cell types and may modulate the effects of epithelial-mesenchymal transition (EMT) factors in these cancers [9–12]. Although there has been interest in finding the receptor for histatin peptides, limited information has been described. Imamura et al.reported on the ability of histatin-3 (Hst3) to interact with, but not clearly directly bind, the chaperone protein HSC70. No other mammalian cellular interactors are known to explain the cell migration and wound healing effects of Hst1 [13,14]. Other clues to a potential receptor include the sensitivity of the effects of histatins to pertussis toxin application, suggesting at least a downstream functional role of a G protein–coupled receptor (GPCR) [7–9].
Given our interest in histatins, we undertook a receptor screening assay to search for a receptor for histatin peptides. This screening assay identified TMEM97 as a putative partner for Hst1. We utilized multiple methods in vetting this interaction, including radioligand binding, coimmunoprecipitation, ELISA assay, direct binding analysis using surface plasmon resonance (SPR), circular dichroism (CD), immunolocalization, and functional assays to validate Hst1 as a binding partner of TMEM97, and identify an endogenous secreted ligand for TMEM97. All of these experiments described herein established TMEM97 as a mammalian histatin receptor. Moreover, we demonstrate that the pro-migratory effects of Hst1 are abrogated by knockdown (KD) of TMEM97. These results have broad importance to understanding how histatins promote cellular migration, identifying an endogenous ligand for TMEM97, and improving our understanding of wound healing and cellular migration.
Results
Hst1 Binds TMEM97
Screening for potential receptors for Hst1 was carried out at the University of North Carolina (UNC) Psychoactive Drug Screening Program (PDSP) using radioligand binding assays on an existing library of overexpressed cell lines of various pharmacologically important receptors. Binding of synthetic Hst1 with TMEM97 containing HEK293T membranes was identified in a primary binding screen at 10 μM of Hst1 and confirmed with a secondary dose–response binding assay using the same radioligand. Results were compared with a reference inhibitor, haloperidol, over a range of doses as described [15], and overlaid dose–response curves of the confirmatory radioligand binding assay test are shown in Fig. 1A. The determined Ki value of the Hst1 was 239 nm, while that of haloperidol was 44 nm. This encouraging Hst1 Ki value motivated us to design and carry out further validation assays.
We then performed a coimmunoprecipitation assay to determine whether the potential interaction between Hst1 and TMEM97 was reproducible at the cellular level. After exogenous application of Hst1 to human corneal epithelial (HCE) cells, cell lysates were obtained and immunoprecipitation with a TMEM97 antibody followed by immunoblotting with an Hst1 antibody demonstrated that Hst1 was coprecipitated with TMEM97 (Fig. 1B). Interestingly, two bands (one below 10 kDa and one just below 15 kDa) were noted on co-IP. These bands may represent monomeric and multimeric versions of Hst1 and are similar to what has been seen with western blotting of Hst1 containing samples in the past [16]. These results suggest that the cell-free findings of this interaction (radioligand binding assay in cell membrane preparations) are demonstrable in the physiologic cellular environment. These results were confirmed with Hst1 antibody immunoprecipitation and blotting with anti-TMEM97 antibody (Fig. S1). In addition, proteomic analysis was performed using the gel fragment taken from the co-IP (precipitated with anti-Hst1 and blotted with anti-TMEM97) and confirmed the presence of TMEM97 (data not shown). Enzyme-linked immunosorbent assay (ELISA) testing to detect bound Hst1 on plates coated with TMEM97 was also performed and confirmed binding between Hst1 and TMEM97 (Fig. 2).
Both the radioligand binding assay and co-IP were done in cellular environments with other cellular components present with the possibility of having some other mediating partners. Hence, we investigated direct binding analysis of Hst1 with purified recombinant TMEM97 proteins in vitrousing the SPR technique. SPR testing was performed with the highly sensitive SPR instrument Biacore 8K and demonstrated that Hst1 selectively bound to full-length TMEM97, which was expressed in a eukaryotic system (HEK293 cells; Fig. 1C).
Subsequent testing with a GST-tagged, wheat germ–derived, C-terminal domain of TMEM97 (108-176) determined that Hst1 bound to the C-terminal region of TMEM97 with similar binding affinity as the full-length protein (Fig. 1E). As shown in Table 1, Hst1 contains many charged residues, which could cause nonspecific charge–charge interactions even though 150 mM salt was present in the SPR binding buffer to reduce this potential nonspecific interaction. A scrambled peptide control version of Hst1, Hst1-scrambled peptide (SP), contains the exact same amino acid residues as Hst1 but with different order. Hst1SP did not exhibit binding to either full-length TMEM97 or TMEM97 (108-176; Fig. 1D,,F),F), indicating the observed binding interaction between Hst1 and TMEM97 was unlikely to be derived by nonspecific charge–charge interactions. These results suggest that there is a specific and direct binding of Hst1 with the C-terminal domain of TMEM97.Table 1.Sequences of synthetic TMEM97, Hst1 and Hst1SP used in experimentation
Name | Sequence |
---|---|
TMEM97 (108-176) | MTTLIPILSTFLFEDFSKASGFKGQRPETLHERLTLVSVYAPYLLIPFILLIFMLRSPYYKYEEKRKKK |
Histatin-1 (Hst1) | DpSHEKRHHGYRRKFHEKHHSHREFPFYGDYGSNYLYDN |
Hst1SP | HYHKFHRYYDPGSNLYKEHNHGFHHGYKDEFRREpSRDS |
Sequences of synthetic TMEM97, Hst1 and Hst1SP used in experimentation
Subsequently, we generated synthetic C-terminal TMEM97 (108-176) and found that C-terminal synthetic TMEM97 (108-176) bound Hst1 with similar affinity to full-length and GST-tagged recombinant TMEM97 (108-176; Fig. 2A). As expected, Hst1SP did not bind to the synthetic TMEM97 (108-176; Fig. S3). In order to compare side by side, overlaid steady-state affinity fitting curves of Hst1 binding to a full-length TMEM97 (black), a GST-TMEM97(108-176) (red) and a synthetic TMEM97(108-176) (green) are shown in Fig. 2B), and their determined KD values are 5.3, 6.6 and 6.3 μm, respectively. We then tested the hypothesis that interaction between Hst1 and TMEM97 could affect their structures upon binding. We examined the secondary structure of Hst1, Hst1SP, and TMEM97 (108-176) in solution using CD measurements (Fig. 2C). We noted that Hst1 and scrambled Hst1SP alone are mostly disordered (over 50%) with some degree of β-strand (light and dark blue lines, respectively, in Fig. 2C, while TMEM97 (108-176) contains mainly α-helical (46%) and 23% β-strand regions with ~31% disordered (14% turns and 17% unordered) regions (Fig. 2D). According to CD spectra comparison (pink line vs black line), there appeared to be slightly more α-helical secondary structures formed upon Hst1 binding to TMEM97 (108-176) than TMEM97 (108-176) alone, reducing turns region from 14% to 8% while β-strand and unordered regions remain similar. The scrambled peptide Hst1SP did not induce increased secondary structural elements, unlike Hst1 (green line in Fig. 2C). Simple sum (orange line) of CD spectra of TMEM97 (108-176) alone and Hst1 alone was demonstrably different from Hst1 bound to TMEM97 (108-176). CD data analysis using DichorWeb revealed that disordered regions (turns plus unordered) of the Hst1-TMEM97 (108-176) complex are lower than each of them alone. These results suggested that not only does Hst1 bind to TMEM97, but the interaction may further induce secondary structures.
Hst1 and TMEM97 co-localize in cellular environments
In order to determine whether the binding assay results were relevant to normal cellular conditions and function, we performed several assays to confirm the existence and relevance of an interaction between Hst1 and TMEM97. We tested whether Hst1 could be internalized into HCE cells and whether localization of internalized Hst1 could colocalize with TMEM97. Figure 3A demonstrates the results of these experiments. Alexa-488 coupled synthetic Hst1 was applied exogenously and noted to be internalized into HCE cells at 24 h after exposure, with relative enrichment of localization to the peri-nuclear area. Subsequent immunolocalization of exogenously applied Hst1 to HCE cells and visualization with an ER staining agent demonstrated good colocalization (Fig. 3B). Finally, coimmunolocalization of exogenously applied Hst1 with TMEM97 demonstrated significant overlap (Fig. 3C). These results suggest that Hst1 is localized to the area where TMEM97 is thought to have a functional role and that the previously described binding assays may have a physiological correlate in live cells. In addition, we performed Hst1 localization by immunofluorescence after exogenous application of Hst1 in cells pretreated with escalating concentrations of 1,3-di-o-tolylguanidine (DTG) and noted that Hst1 localization is reduced by pretreatment with DTG, correlating well with radioligand binding assay results (Fig. S4).
TMEM97 is required for migration promotion by Hst1
Testing was then performed to determine whether the known functions of Hst1 on HCE or HeLa cells were dependent upon TMEM97 expression, including cell migration and wound healing. In order to assess the importance of TMEM97 in the known functions of Hst1, we performed a siRNA KD of TMEM97 in HCE and HeLa cells (Fig. 4). Internalization and localization of Hst1 in KD cells versus control cells were then tested (Fig. 4C). Notably, KD of TMEM97 significantly disrupted internalization and/or localization of Hst1 in HCE KD cells. Boyden chamber-based cell migration assays were performed in HCE and HeLa cells with and without siRNA KD of TMEM97 and demonstrated that, as predicted, Hst1 treatment caused a dose-dependent increase in cellular transmigration toward a stimulus (2% FBS). KD of TMEM97 in HCE and HeLa cells abolished this response to Hst1, suggesting that transmigration acceleration in response to Hst1 is dependent upon the presence of TME97 (Fig. 4D–-F).F). Subsequently, we tested whether Hst1-dependent increases in wound healing of HCE cells, using a standard scratch assay, were dependent upon TMEM97. In order to do this, we generated a shRNA KD of TMEM97 in HCE cells (Fig. 4G). Similarly, we noted the predicted increases in wound healing rates in response to Hst1 in HCE cells and shRNA KD of TMEM97 abolished this responsiveness (Fig. 4H–-I).I). Figure S5demonstrates a similar necessity of TMEM97 in HCE cells to increase rates of scratch closure in response to Hst1 using a siRNA-mediated KD. As the mechanism for Hst1 induced wound healing and epithelial cell migration has been the subject of much study and is still unknown, this finding suggests a novel potential pathway/mechanism for this phenomenon.
Discussion
This study represents the demonstration of an endogenous ligand for TMEM97 and a mammalian receptor for Hst1. We discovered this novel ligand–receptor relationship through radioligand binding assay screening and confirmed these results by multiple different biochemical/biophysical methods of measuring protein–protein interaction. We also confirmed the necessity of TMEM97 for the wound healing activity of Hst1 peptides and found potential domains for interaction between these two proteins. These results may impact understanding of processes including wound healing, cholesterol processing, pathogen processing, and mucosal immunity.
TMEM97 was recently reported to be the elusive S2R [1]. S2R is the target of numerous pharmacologic ligands and is targeted for multiple purposes including cancer, schizophrenia, Alzheimer’s disease, aging and mitochondrial disorders, and multiple sclerosis [2]. TMEM97 is an intrinsic ER protein involved in cholesterol and lysosomal processing that has been implicated in cell migration and interacts with NPC cholesterol transporter 1 protein, NPC1 [1,2]. NPC1 is critically important in cholesterol processing, pathogen processing, autophagy, and apoptosis [3–5]. Reduction of TMEM97 levels in NPC1 mutant cells leads to relative normalization of free cholesterol levels, a critical feature of NPC disease, suggesting a therapeutic role for modulation at the TMEM97 protein for NPC disease [17].
S2R targeting agents have also been implicated in migration, caspase-mediated apoptosis, autophagy, reactive oxygen species generation, and mitochondrial stability [2]. One of the most important effects of the S2R/TMEM97/MAC30 is the promotion of cell migration and association with malignancies [18–22]. TMEM97 up- and downregulation has been seen in multiple tumor types, with upregulation seen in many cancers, and downregulation seen in renal and pancreatic cancers [23]. Interestingly, changes in cancer cell motility, invasiveness, and deregulation of epithelial-mesenchymal transition markers have been seen with derangement of TMEM97 levels [18,19,23,24]. While multiple actions and pharmacologic modulators have been tied to TMEM97/S2R and great importance has been placed on TMEM97, no endogenous ligand is known [1,2].
Histatins are an important class of endogenous AMPs. Other exemplary AMPs include LL-37 and β-defensins [6]. Histatin peptides are a histidine-rich family of peptides, arising from two genes (HTN1 and HTN3). Histatins were first described as antifungal agents in the saliva, but have since been found to have antiviral, antibacterial, wound healing, and even antiinflammatory activities [7–9]. Histatins, particularly Hst1 are thought to be the primary wound healing agents in the oral mucosa and promote cell migration and modulate cellular adhesion in epithelial, stromal, and vascular endothelial cells [7–9]. Migration induced by Hst1 in some cell types may involve endosomal protein recruitment and MAP kinase signaling changes [7–9]. Interestingly, it was recently reported that Hst1 is readily taken up into human buccal epithelial carcinoma cells and localized to the ER and mitochondria [25].
Histatins may be overexpressed in several cancer cell types and may modulate effects of EMT factors in some cancer cell types [9–12]. Moreover, a number of antimicrobial peptides genes (HTN1, HTN3, STATH, MUC7), with similar chromosomal localization (chr 4; 4q13.3) are seen to be significantly overexpressed in the peri-tumor area in papillary thyroid cancer samples, suggesting some potential interaction of these AMPs with cancerous lesions [26]. Hst5 also has sequence homology with CCL28 a chemokine with antimicrobial activity that has been tested as an adjuvant for HIV1 vaccine development and is upregulated in multiple cancers [27].
While Imamura et al. have reported on the ability of Hst3 to interact with, but not clearly directly bind, the chaperone protein HSC70, no other mammalian cellular interactors are known to explain the migratory effects of Hst1 [13,14]. Interestingly, HSC70 may be a modulator of NPC and levels of HSP70/HSC70 contributing to stabilization or destabilization of NPC1 levels [28].
Other clues to a potential interaction among histatins, TMEM97, and NPC1 are also seen in disparate reports of these previously unassociated proteins being involved in viral processing, infectivity, and invasion. HIV1 infected patients have been reported to have decreased Hst5 levels and increases in Candida albicans infections [9,29]. Moreover, NPC1 has been identified as the Ebola virus receptor. The common tie among these observations could be the importance of cholesterol processing in many viral infections [30].
While we believe it is exciting and impactful to identify an endogenous receptor for Hst1 and an endogenous ligand for TMEM97, it is not yet clear whether all of the actions of histatin peptides are explained by an interaction with TMEM97, or whether all the actions of TMEM97 are driven by histatins. It is possible that the associations of both of these proteins with phenotypic cellular changes are contextual and depend on tissue type, environmental conditions or other factors. This study does not answer whether a GPCR still exists to underpin some actions of histatin peptides and whether such a receptor is up or downstream of TMEM97. Finally, the interaction we describe could be one of the multiple interactions, and future experiments are still needed to solve the structures of TMEM97 and Hst1 alone and together.
In conclusion, Hst1 is an endogenous ligand for TMEM97 and TMEM97 is a mammalian receptor for Hst1. Moreover, TMEM97 is necessary for transduction of the epithelial migration promoting effects of Hst1. These results help to resolve the search for an Hst1 receptor and broaden our understanding of physiological interactors for TMEM97. Implications for these findings may be found in our understanding of cholesterol processing, NPC disease, wound healing, and cell migration.